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KMID : 0545120000100010048
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 1 p.48 ~ p.50
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The Succinate
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KIM YOUNG-JAE
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Abstract
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The energetics at the succinate:quinone oxidoreductase segment of V alginolyticus was studied using a fluorescence quenching technique with inside-out membrane vesicles. A transient generation of the membrane potential (inside-positive) and △pH (inside-acidic) occurred in the presence of KCN and succinate when ubiquinone-1 (Q1) was added. The membrane potential (△ψ) generated by the succinate: quinone oxidoreductase segment was completely collapsed by the protonophore carbonylcyanide m-chlorophenylhydrazone (CCCP) and the membrane permeable anion SCN whereas the △pH was completely collapsed by CCCP and (NH_4)_2SO_4. From these results, it was concluded that the succinate: quinone oxidoreductase segment as well as quinol oxidase [1] in the respiratory chain of V. alginolyticus generated H^+ electrochemical potential.
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